Brazilian Journal of Medical and Biological Research (2005) 38: 577-582Effect of a rehydration sports drink on urolithiasis
ISSN 0100-879X
Effect of an isotonic rehydration sports drink and exercise on urolithiasis in rats
. Bergamaschi2, 2Disciplina de Fisiologia Cardiovascular e Respiratória,
G.S. di Marco1, Departamento de Farmacologia,
C.V. Razvickas1, Universidade Federal de São Paulo, São Paulo, SP, Brasil
and N. Schor1
The objective of the present study was to evaluate the role of physical
exercise as well as the influence of hydration with an isotonic sports
drink on renal function in male Wistar rats. Four groups were studied
over a period of 42 days: 1) control (N = 9); 2) physical exercise (Exe,
N = 7); 3) isotonic drink (Drink, N =

; 4) physical exercise + isotonic
drink (Exe + Drink, N =

. Physical exercise consisted of running on
a motor-driven treadmill for 1 h/day, at 20 m/min, 5 days a week. The
isotonic sports drink was a commercial solution used by athletes
for rehydration after physical activity, 2 ml administered by
gavage twice a day. Urine cultures were performed in all animals.
Twenty-four-hour urine samples were collected in metabolic cages
at the beginning and at the end of the protocol period. Urinary
and plasma parameters (sodium, potassium, urea, creatinine, calcium)
did not differ among groups. However, an amorphous material was
observed in the bladders of animals in the Exe + Drink and Drink
groups. Characterization of the material by Western blot revealed the
presence of Tamm-Horsfall protein and angiotensin converting en-
zyme. Physical exercise and the isotonic drink did not change the
plasma or urinary parameters measured. However, the isotonic drink
induced the formation of intravesical matrix, suggesting a potential
lithogenic risk.
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